Formulation of Cantigi (Vaccinium varingiaefolium Blume Miq.) Leaf Extract-Loaded Gelatin Nanoparticles as Anti-Breast Cancer In vitro, Edition 1

This book is my beginning research to introduce the Cantigi plant (Vaccinium varingiaefolium Blume Miq). The plants grow well surrounding the crater of Mount Tangkuban Parahu, Bandung, Indonesia, and make it beautiful. This plant is in the same genus Vaccinium as the famous, researched, and traded Bilberry plant. On this occasion, I explored the in vitro potential of Cantigi leaf extract as anti-breast cancer using BSLT, T47D cells, and MCF7. Tests were also carried out on normal Vero cells to see whether the effect of the extract was safe on normal cells. Cantigi leaf extract was then analyzed using GC-MS and LC-MS/MS methods to reveal the content of volatile and nonvolatile compounds, in addition to the screening method. In this research, efforts to increase cytotoxic activity and extract stability are using gelatin nanoparticle technology. Then, experiments were designed and conducted with three variables, and one of the gelatin nanoparticle compositions provided the most potent activity against a cancer cell line. The nanoparticles were then characterized, including their release profile at the pH of body fluids. This book still has room for suggestions and development to improve. Finally, I hope this book can contribute to the research of Cantigi as one of the natural medicine sources.